Thermo Scientific™ Colonnes RP DNAPac™

Beschreibung

Obtenez des séparations supérieures des oligonucléotides en phase inverse en utilisant les colonnes HPLC RP Thermo Scientific™ DNAPac™. La chimie unique des colonnes est conçue pour l’analyse d’oligonucléotides et de fragments d’ADN/ARN double brin (db) à l’aide de la méthode LC-UV ou LC-MS. La chimie de ces colonnes offre d’excellentes performances sur une large gamme de pH, de températures et de compositions de phase mobile. En outre, la large taille des pores de la résine offre une excellente séparation des grands acides nucléiques double brin, jusqu’à 10 000 paires de base.

DNAPac RP reversed phase (RP) column is based on spherical wide-pore 4 μm polymer resin specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is highly stable to extreme pH (0-14), and temperature (5-110°C) and fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of small to large nucleic acids with low carry-over. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments:

• Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both oligonucleotides and double-stranded nucleic acids
• High resolution and high throughput
• Excellent MS compatibility
• Wide operating pH range (0-14) and high temperature stability (up to 110°C)
• Biocompatible hardware to eliminate analyte absorption