Invitrogen™ DynaGreen™ Protein A Magnetic Beads

Description

DynaGreen Protein A Magnetic Beads are intended for use in immunoprecipitation (IP) applications while also being an environmentally sustainable choice. They are microplastic-free submicron magnetic beads that provide reproducible isolation of target proteins in a simple and efficient workflow, which can be performed manually or automated on KingFisher™ instruments.

Benefits of DynaGreen Protein A Magnetic Beads include:

• High performance—reproducible IP with high-yield and low non-specific binding
• Reduced environmental impact—non-microplastic bead core and holistic sustainable product design
• Flexible—direct and indirect immunoprecipitation helps ensure isolation of non-abundant proteins
• Automation ready—easy automation for high-throughput needs on the Kingfisher Sample Purification Systems

Simple, gentle, and efficient manual separation

DynaGreen Protein A Magnetic Beads are microplastic-free superparamagnetic beads with recombinant protein A (∼45 kDa) covalently coupled to the surface. They were developed for convenient IP of target proteins when the IP antibody interacts strongly with Protein A (see subclass specificity in the manual). The submicron bead size (∼250 nm) provides low sedimentation and a large target capture surface area. The method is a simple bind-incubate-elute protocol with no pre-clearing required. This results in an efficient high-yield isolation of target protein by direct or indirect immunoprecipitation in less than 80 minutes.

The magnetic separation technology used by DynaGreen Protein A Magnetic Beads is rapid and gentle, thereby reducing physical stress to the target proteins. Furthermore, the non-porous nature of the beads lowers the risk of antibodies being trapped and becoming inaccessible. This helps to minimize the amount of antibody required to produce accurate, and reproducible results. The yield and purity of the isolated target protein are highly compatible for use in western blotting and mass spectrometry. The product manual below includes a list of required reagents, materials, and details of compatible buffer recipes.

Rapid and reproducible automated workflow

The DynaGreen magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems or liquid handlers.

The automated protocol can be performed in just 40 minutes with up to 96 samples per run, which helps reduce hands-on time while maintaining the same reproducible high target yield and low non-specific binding compared to the manual protocol.

A certified choice for environmentally sustainable research

Microplastic-free DynaGreen Protein A Magnetic Beads are designed for both high performance in immunoprecipitation workflows and lower environmental impact. They provide exceptional purity and high yield of target proteins compared to other magnetic beads in both direct and indirect immunoprecipitation workflows. At the same time, the product boasts an extensive list of third-party ACT-label-certified sustainable product design elements by incorporating principles of green chemistry and green engineering.

Choosing DynaGreen Protein A Magnetic Beads can help reduce the environmental impact of research without compromising on results.

Learn more about our DynaGreen magnetic beads and KingFisher Sample Purification Systems:

• Alternative sustainable products: DynaGreen Protein A/G (Cat. No. 80106G) and DynaGreen Capture-Select anti-FC (multispecies) (Cat. No. 80107G)
• DynaGreen magnetic beads sustainability
• DynaMag magnets for magnetic bead separations
• KingFisher automation protocols
• KingFisher Flex instrument video