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Invitrogen™ Cortisol Competitive Human ELISA Kit
Competitive ELISA
Marque: Invitrogen™ EIAHCORX10
Code nomenclature Nacres: NA.84
Informations supplémentaires : Poids : 2.00000kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Human Cortisol ELISA quantitates Cortisol in human serum,plasma,urine,saliva,dried fecal extracts or or cell culture medium.
Principle of the method
The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts,saliva,urine,serum,plasma,and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma,and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Spécification
100-3200 pg/ml | |
Kit ELISA compétitif pour cortisone | |
HRP | |
Kit ELISA | |
10 x 96 dosages | |
ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes | |
8,1% | |
Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives | |
RUO | |
Extrait fécal, 50 μl ; plasma, 1 μl ; salive, 12,5 μl ; sérum, 1 μl ; surnageant, 50 μl ; urine, 6,25 μl | |
Humaine | |
1 h 30 min |
17,3 pg/ml | |
Dosage multiplex sur la plateforme Luminex | |
Fluorescent | |
Extraits fécaux, plasma, salive, sérum, surnageant, Urine | |
Humaine | |
Lecteur de microplaques colorimétriques | |
8,8% | |
HRP | |
Inflammation | |
-20°C | |
1 h 20 min |