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Invitrogen™ VCP Monoclonal Antibody (Hs-14)
Mouse Monoclonal Antibody
Marque: Invitrogen™ MA110383
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Description
This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein. For ICC staining, membrane permeabilization with acetone is essential.
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Spécification
| VCP | |
| Monoclonal | |
| 1 mg/mL | |
| TBS with 15mM sodium azide; pH 8.0 | |
| P55072, Q01853 | |
| VCP | |
| Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. | |
| 100 μg | |
| Primary | |
| Human, Mouse | |
| Antibody | |
| IgM |
| Flow Cytometry, Western Blot, Immunocytochemistry | |
| Hs-14 | |
| Unconjugated | |
| VCP | |
| 15S Mg(2+)-ATPase p97 subunit; 3110001E05; AAA ATPase p97; ALS14; CDC48; cell division cycle gene CDC48; CMT2Y; epididymis luminal protein 220; epididymis secretory protein Li 70; HEL-220; HEL-S-70; homolog of yeast cdc48; IBMPFD; IBMPFD1; Inv protein; or HEL-S-70; p97; p97 atpase; p97/VCP; Protein CDC48; si:ch211-113n10.2; TER ATPase; TERA; transitional endoplasmic reticulum ATPase; valosin containing protein; valosin-containing protein; Vcp; wu:fd16d05; wu:fj63d11; yeast Cdc48p homolog | |
| Mouse | |
| Sequential Chromatography | |
| RUO | |
| 269523, 7415 | |
| 4°C, do not freeze | |
| Liquid |
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