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TaqMan® Dosage d’expression génétique VIC TaqMan™
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Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

427.00€ - 1158.00€

Spécification

Méthode de détection Amorce-sonde
Format Tube
GC-Rich PCR Performance Élevé
Méthode PCR qPCR
Vitesse de réaction Rapide
Consulter d'autres spécifications

Alert:

Ce produit nécessite une configuration supplémentaire

Veuillez commander les dosages pour l’expression génétique Applied Biosystems™ TaqMan™ via l’outil de recherche d’expression génétique.


Produits 3
Code produit Marque Quantité Prix Quantité et disponibilité  
Code produit Marque Quantité Prix Quantité et disponibilité  
11927021
GREENER_CHOICE
Afficher les documents Détails de la promotion
TaqMan®
4448489
S (360 reactions), made to order
427.00€
1 pièce
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11957021
GREENER_CHOICE
Afficher les documents
TaqMan®
4448490
M (750 reactions), made to order
560.00€
1 pièce
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11947021
GREENER_CHOICE
Afficher les documents
TaqMan®
4448491
L (2900 reactions), made to order
1158.00€
1 pièce
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Description

Description

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
Accurate—identify small fold-changes with high accuracy of quantitation
Extensive content—over 1.8 million predesigned assays available for over 25 different species
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix:TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

Spécification

Spécification

Amorce-sonde
Élevé
Rapide
Emballage durable
60 X
Tube
qPCR
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Dosage d’expression génétique
Vidéos
FDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.