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R&D Systems™ Recombinant Human VEGF 165 Protein
Marque: R&D Systems™ BT-VEGF-250
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Description
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.50-12.0 ng/mL.Spécification
| NP_001165097.1 | |
| Lyophilized from a 0.2 μm filtered solution in Sodium Acetate. | |
| M.W. Observed: 19-21 kDa, under reducing conditions., M.W. Theoretical: 19.2 kDa (monomer) | |
| E. coli-derived human VEGF protein Ala27-Arg191 | |
| <0.01 EU per 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Bioactivity | |
| 7422 | |
| 250 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. | |
| MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor | |
| Reconstitute the 10 μg size at 100 μg/mL in sterile deionized water. Reconstitute all other sizes at 500 μg/mL in sterile deionized water. | |
| VEGF |
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