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enQuireBio™ Recombinant Human MEK1 Protein
A cDNA sequence encoding the MEK1 was constructed and used to recombinantly synthesize the protein.
Marque: enQuireBio™ QP10784-5ug
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Spécification
| MEK1 Protein | |
| Research Use Only | |
| ∼125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30C in a reaction containing 100microm ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. | |
| Human | |
| His | |
| MEK1 is supplied at a concentration of in 40mM Tris, pH 8.0, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35. |
| 5 μg | |
| < 1.0 EU per ug protein as determined by the LAL method. | |
| Recombinant Protein | |
| Baculovirus | |
| MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGV | |
| Greater than 90% as determined by SDS-PAGE. |
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