Cet article est supprimé, vous trouverez le remplacement et les alternatives dans les onglets respectifs ci-dessous s'ils ont été identifiés Voir les produits de remplacement
»
Get rapid and efficient protein transfer at your fingertips with the Thermo Scientific™ Pierce™ Power Blotter.
The Thermo Scientific Pierce Power Blotter is designed specifically for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes.
The Pierce Power Blotter replaces the Pierce G2 Fast Blotter and features an integrated power supply optimized to deliver consistent, high-efficiency protein transfer when used with commonly used precast or homemade gels (SDS-PAGE) and nitrocellulose or PVDF membranes. The Power Blot cassette enables the simultaneous transfer of up to four mini-sized gels or two midi-sized gels. The Power Station component of the Power Blotter has an easy-to-use color LCD touchscreen interface and pre-programmed transfer methods for different numbers and sizes of gels and ranges of protein molecular weight. The easy-touch programming feature allows custom transfer settings to be quickly created, saved and run.
The Pierce Power Blotter achieves highly efficient transfer in less than 10 minutes when used with Pierce 1-Step Transfer Buffer. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional buffers.
Highlights:
Efficient – achieve high transfer efficiency with a broad range of protein sizes (10-300kDa) compared to conventional semi-dry or wet (tank) transfer methods
Fast – transfer proteins in 5 to 10 minutes when used with 1-Step Transfer Buffer
Integrated power supply – seamless operation between control unit and cassette provides consistent high efficiency protein transfer
Easy-touch programming – access pre-programmed transfer methods or create, save and run customized transfer methods
Convenient – simultaneously transfer 1 to 4 mini gels or 1 to 2 medium-sized gels
Versatile – use with Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods
Upgrade-ready – adding the Pierce Power Stain Cassette activates the pre-loaded software making this unit a fully functional Pierce Power System with gel staining and blotting capabilities.
Includes:
System consists of the Pierce Power Station and Pierce Power Blot Cassette; kit includes Western Blot Roller, Power Cord with C/13 Connector, and Quick Start Guide
Unit ships with North American power cord; country-specific power cords are also available
Applications:
Semi-dry Western blot transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in about 10 minutes using optimized transfer buffer
Optional:
Pierce Power Stain Cassette (22836) - activates pre-loaded software to enable electrophoretic protein staining
Product Details:
Traditional wet blotting techniques require transfer times of one hour to overnight to achieve good transfer efficiency. When used in conjunction with Pierce 1-Step Transfer Buffer, the Pierce Power Blotter provides efficient protein transfer in 5-10 minutes with results equivalent to, or better than, traditional Western blot transfer techniques. This system also eliminates the need for gel pre-equilibration. This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buffer and increasing the current [amps (A)/cm2] flowing through the transfer stack. The Pierce Power Blotter can also be used for standard 30- to 60-minute semi-dry transfer protocols with Towbin buffer (Towbin et al., 1979).
Pierce™ Power Blotter Welcome Pack, SDS-PAGE Gels Compatibility, Horizontal Running Dimension, Semi-Dry Mode of Transfer, 100 to 240V, 50/60Hz, 168W Electrical Requirements, Use With iBlot™, For Protein Assays and Analysis
Pierce™ Power System
Pierce power station, pierce power blot cassette, western blot roller, power cord with C/13 connector, quick start guide, pierce 1-Step transfer buffer, 1L western blotting filter paper, 7 x 8.4cm, 100 sheets
100 to 240 V 50/60 Hz 168 w
168 w
Protein assays and analysis, protein gel electrophoresis
Clients qui ont consulté cet article ont également consulté
This information does not imply a recommendation or representation of any kind and any action taken upon the information provided is strictly at your own risk. The products shown have not been qualified by Fisher Scientific for any particular purpose, application or use and it is the responsibility of the purchaser to ensure that the performance of the product is suitable for the purchasers specific use or intended application.
