Invitrogen™ Kit ELISA pour MIP-3 alpha / CCL20 humaine dans les lysats cellulaires

Description

The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu MIP-3α. Principle of the method The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La protéine inflammatoire des macrophages-3-alpha (MIP-3 alpha), également appelée CCL20, est exprimée dans le foie, les poumons, les ganglions lymphatiques et les lymphocytes du sang périphérique. La MIP-3 alpha est fortement régulée par les signaux inflammatoires et régulée à la baisse par la cytokine anti-inflammatoire IL-10. La MIP3alpha signale par le biais du récepteur couplé aux protéines G, CCR6, et agit comme chimiotactique pour les lymphocytes et les cellules dendritiques.