Thermo Scientific™ Kit de synthèse de l’ADNc premier brin Maxima pour la RT-qPCR

Description

Le kit de synthèse d’ADNc premier brin Maxima pour la RT-qPCR est un système pratique optimisé pour la synthèse d’ADNc dans les applications de RT-PCR quantitatives (RT-qPCR) en deux étapes. Le kit utilise la transcriptase inverse (RT) Maxima, une enzyme avancée dérivée par évolution in vitro de la RT M-MuLV. L’enzyme présente une grande thermostabilité, une robustesse et un taux de synthèse d’ADNc plus élevé par rapport à la RT M-MuLV de type sauvage.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 μg) at elevated temperatures (42–65°C). The synthesis reaction can be completed in 15–30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase also contains a novel double-strand specific DNase engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Features of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR include:

• High yields of full length cDNA up to 20 kb
• Efficient cDNA synthesis at wide temperature range (42–65°C)
• Increased synthesis rate—complete cDNA synthesis in 15–30 minutes
• High sensitivity and specificity
• Integrated gDNA removal step with dsDNase kit

Maxima First Strand cDNA Synthesis Kit with dsDNase contains:

• Maxima Enzyme Mix
• 5X Reaction Mix
• nuclease-free water

Kit with dsDNase also contains

• dsDNase
• 10X dsDNase Buffer

Applications

• 2-step RT-PCR
• 2-step RT-qPCR

Additional information about reaction components

The Maxima Enzyme Mix contains Maxima Reverse Transcriptase and RiboLock RNase Inhibitor. The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C.

5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers. Nuclease-free water is provided for reaction set-up and dilution of sample RNA.

The absence of endo- and exo-deoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.