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Ly-6G Monoclonal Antibody (1A8-Ly6g), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
175.00€ - 328.00€
Spécification
Antigène | Ly-6G |
---|---|
Clone | 1A8-Ly6g |
Concentration | 1 mg/mL |
Applications | Flow Cytometry, Functional Assay |
Classification | Monoclonal |
Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
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Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
15507836
|
Invitrogen
16-9668-82 |
100 μg |
175.00€
100µg |
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15517836
|
Invitrogen
16-9668-85 |
500 μg |
328.00€
500µg |
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Description
Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown. Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity. Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis and depletion of neutrophils. Moreover, this antibody has been reported to have antagonist activity. (Fluorochrome-conjugated 1A8-Ly6g is recommended for use in flow cytometry.). Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.Spécification
Ly-6G | |
1 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G | |
Ly6g | |
Primary | |
4° C | |
Ly6g |
1A8-Ly6g | |
Flow Cytometry, Functional Assay | |
Functional Grade | |
Rat | |
Mouse | |
P35461 | |
546644 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.