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Ly-6G Monoclonal Antibody (1A8-Ly6g), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Marque: Affymetrix eBioscience 11-9668-82
Code nomenclature Nacres: NA.41
Informations supplémentaires : Poids : 0.09500kg
Description
Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown. Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity. Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.Spécification
Ly-6G | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P35461 | |
Ly6g | |
Affinity chromatography | |
RUO | |
546644 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
1A8-Ly6g | |
FITC | |
Ly6g | |
Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.