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Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555, Invitrogen™
Goat Polyclonal Secondary Antibody
Marque: Invitrogen A21429
Description
To minimize cross-reactivity, these goat anti-rabbit IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 555 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity.
To minimize cross-reactivity, these goat anti-rabbit IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 555 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 μg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications. Product will be shipped at Room Temperature.![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Spécification
Rabbit IgG (H+L) Highly Cross-Adsorbed | |
Polyclonal | |
Alexa Fluor 555 | |
Goat | |
purified | |
RUO | |
Rabbit | |
Liquid |
Flow Cytometry, Immunocytochemistry | |
2 mg/mL | |
PBS with 5mM sodium azide; pH 7.5 | |
Gamma Immunoglobins Heavy and Light chains | |
1 mg | |
Secondary | |
4° C, store in dark | |
IgG |
Sécurité et traitement
- Anti-Mouse IgG (Fc specific)
Mention d'avertissement
- Attention
Catégorie de danger
- Sensibilisation cutanée Catégorie 1
Mention de danger
- H317-Peut provoquer une allergie cutanée.
Conseils de prudence
- P261-Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/ aérosols.
- P272-Les vêtements de travail contaminés ne devraient pas sortir du lieu de travail.
- P280-Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/ du visage.
- P302+P352-EN CAS DE CONTACT AVEC LA PEAU: Laver abondamment à l'eau/.
- P333+P313-En cas d'irritation ou d'éruption cutanée: consulter un médecin.
- P362+P364-Enlever les vêtements contaminés et les laver avant réutilisation.
Informations supplémentaires
- MIXTURE LIST-Contient: peroxidease, 2-methyl-4-isothiazolin-3-one