Invitrogen™ Kit ELISA de GLP-1 7-36a humain

Description

The Human GLP-1 7-36a ELISA quantitates Hu GLP-1 7-36a in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GLP-1 7-36a. Principle of the method The Human GLP-1 7-36a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Le glucagon est une hormone peptidique sécrétée par les cellules alpha des îlots de Langerhans du pancréas. Le peptide est synthétisé comme un précurseur de 180 acides aminés qui est converti en glucagon par la prohormone convertase 2 (PC2). Le rôle principal du glucagon est la gluconéogenèse et la glycogénolyse. Il stimule la libération de glucose en convertissant le glycogène dans le foie et maintient également l’homéostasie du glucose en coordination avec l’insuline.