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STEMCELL Technologies G418
Antibiotic for selecting transfected mammalian cells
Marque: STEMCELL Technologies 03812
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Description
- Antibiotic G418 is an aminoglycoside related to gentamicin and is toxic to bacteria, yeast, higher plants, mammalian cells, protozoans, and helminths
- G418 binds to 80S ribosomes in prokaryotic and eukaryotic cells and inhibits protein synthesis, resulting in cell death
- Resistance to the antibiotic is conferred by aminoglycoside-3-phosphotransferase
- Vectors containing the G418 resistance gene (neo) are commonly used for transfecting eukaryotic cells, with selection of transfected cells achieved by culturing them in the presence of G418
- Cells may exhibit variable resistance to the antibiotic and can take up to a week to die
- The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), ClonaCell™-CHO CD Medium (Catalog #03815), ClonaCell™-CHO ACF Medium (Catalog #03816), ClonaCell™CHO CD Liquid Medium (Catalog #03817), or other cell culture media
Spécification
| 108321-42-2 | |
| 250 mg |