Invitrogen™ Endocan (ESM1) Mouse ELISA Kit

Description

The Mouse Endocan (Ms ESM1) ELISA quantitates Ms Endocan in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Endocan. Principle of the method The Mouse Endocan solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Ce gène code une protéine sécrétée qui est principalement exprimée dans les cellules endothéliales des tissus pulmonaires et rénaux humains. L’expression de ce gène est régulée par des cytokines, ce qui suggère qu’il peut jouer un rôle dans les troubles pathologiques dépendant de l’endothélium. La transcription contient plusieurs signaux d’instabilité de polyadénylation et d’ARNm. Deux variantes de transcription codant différentes isoformes ont été trouvées pour ce gène.