CD124 Monoclonal Antibody (X2/45-12), Functional Grade, eBioscience™, Invitrogen™

Description

The X2/45-12 monoclonal antibody reacts with the human IL-4 receptor alpha chain. IL-4 receptor alpha chain (CD124) is crucial for the binding and signal transduction of IL-4. Both IL-4 and IL-13 use the IL-4 receptor alpha chain as their receptor component and activate many common signal pathways. Applications Reported: X2/45-12 has been reported for use in flow cytometric analysis. X2/45-12 has also been reported in neutralization of human IL-4 function. Applications Tested: The X2/45-12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD124 Monoclonal antibody specifically detects CD124 in Human samples. It is validated for Flow Cytometry, Functional Assay