Carbonic Anhydrase IX Monoclonal Antibody (2D3), Invitrogen™

Description

MA5-15737 targets CA9 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-15737 immunogen is purified recombinant fragment of human CA9 expressed in E. Coli. MA5-15737 detects CA9 which has a predicted molecular weight of approximately 50kDa.

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.