Bovine Serum Albumin Polyclonal Antibody, Invitrogen™

Description

Anti-bovine serum albumin (anti-BSA) antibody is a highly purified rabbit IgG fraction that can be used for the detection of BSA in tracer studies or in protease assays. This antibody demonstrates high specificity for BSA and little or no crossreactivity to other serum proteins. Cat. No. A11133 can be used in enzyme and fluorescence immunoassays and blots, cell or tissue immunostaining, and as a precipitating antibody or as an immuno-adsorbant when attached to solid supports. For detection of this rabbit antibody, we offer a variety of second-step reagents labeled with biotin or with a wide range of fluorophores. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. We suggest using serial dilutions from about 1:100 to 1:1,000 to determine the optimal conditions for your experiments.

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.